Total lysates (15ug protein) from 293T and 293T/PARP knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5ug/mL) P3113-06D1. Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody. 1:2000 dilution of HRP anti-β-actin Antibody was used as a loading control (lower). Lane M: MW ladder.